Preparation of megabase-size DNA from plant nuclei
نویسندگان
چکیده
منابع مشابه
Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads.
Conventional methods for preparation of megabase plant DNA require a protoplast preparation step (1—5). Preparation of protoplasts is tedious and time-consuming. In contrast isolation of nuclei from leaf tissue is simple. Many researchers have attempted to prepare megabase DNA from nuclei (e.g., 3-6) , but no success has been reported. As an alternative approach Guidet and Langridge (6, 7) desc...
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A prerequisite for constructing a physical map that spans an entire plant chromosome is isolation of intact chromosome-sized DNA. Two methods are commonly used to isolate megabase (Mb)-sized DNA from plant materials, i.e., from protoplasts (1) or from nuclei (3,5). Isolated protoplasts or nuclei are embedded in low-melting-point agarose to form either plugs or microbeads. DNA molecules are isol...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1995
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1995.07010175.x